Journal: Cell Death & Disease

Article Title: Histone lactylation-driven feedback loop modulates pyrimidine metabolism to promote oral carcinogenesis

doi: 10.1038/s41419-026-08580-w

Figure Lengend Snippet: A UMAP plot of the clustering results for eight major cell types from OLK and OSCC tissues. B Marker genes of each cluster. C UMAP plot showing the cell distribution across all 14 samples. D Reactome pathway analysis of DEGs with gradually increased expression between epithelial cells from OLK and primary OSCC. E Lactate content in normal ( n = 30), OLK ( n = 30), and OSCC ( n = 30) tissues. F Lactate content in HOK, DOK, and OSCC cells (HSC3, HN4, HN6, and CAL27). The representative images (×200) ( G ) and quantification analysis of Pan Kla ( H ) and H3K18la ( I ) protein levels in normal ( n = 30), OLK ( n = 30), and OSCC ( n = 30) tissues were assessed by immunohistochemical staining. Scale bar: 100 μm. ( J ) Pan Kla and H3K18la levels in human HOK, DOK, and OSCC cells (HSC3, HN4, HN6, and CAL27) were analyzed by western blotting assays. Error bars, mean ± SD; * P < 0.05.

Article Snippet: The human OSCC cell lines (HSC3, CAL27 and UPCI-SCC-154) were obtained from the American Type Culture Collection (VA, USA).

Techniques: Marker, Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: Cell Death & Disease

Article Title: Histone lactylation-driven feedback loop modulates pyrimidine metabolism to promote oral carcinogenesis

doi: 10.1038/s41419-026-08580-w

Figure Lengend Snippet: A TK1 mRNA levels in normal ( n = 30) and OSCC ( n = 308) tissues were analyzed using the data from TCGA-OSCC cohort. B Kaplan–Meier survival analysis was performed to analyze overall survival based on TK1 mRNA levels (Low TK1 versus High TK1) in patients with OSCC in TCGA cohort ( n = 308). C TK1 mRNA levels in normal ( n = 30), OLK ( n = 30), and OSCC ( n = 30) tissues were measured using real-time RT-PCR. D – I DOK and CAL27 cells stably expressing NC or sh-TK1 were used to assess the effects of TK1 silencing on cell proliferation. The TK1 protein levels were measured using western blotting assays ( D ). Cell growth was evaluated using CCK-8 assay ( E ) and colony formation assay ( F , G ). The cell cycle distribution was measured using flow cytometry ( H , I ). Each experiment was performed in triplicate. Error bars, mean ± SD; * P < 0.05.

Article Snippet: The human OSCC cell lines (HSC3, CAL27 and UPCI-SCC-154) were obtained from the American Type Culture Collection (VA, USA).

Techniques: Quantitative RT-PCR, Stable Transfection, Expressing, Western Blot, CCK-8 Assay, Colony Assay, Flow Cytometry